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Part:BBa_K5310033:Design

Designed by: Alexandros Liatsos   Group: iGEM24_thessaloniki   (2024-09-30)


sgRNA from FOXP3 gene for CRISPR editing (Oligo 7)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Proper selection of target sequences, so as not to be overlapping, is of paramount significance. Furthermore, restriction enzyme sequences for BsmBI were also taken into account and introduced.


Source

FOXP3 gene

References